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We model the reaction as linear and reversible because covalent binding of NAPQI gradually declines after eight hours[28, 29]

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ERp57 is protective against mutant SOD1-induced cellular pathology in amyotrophic lateral sclerosis

Following 3 hours of feeding the CR mice at the start of the light cycle, all dietary groups underwent a 3 hr fast, followed by cervical dislocation and tissues for molecular analysis were flash-frozen in liquid nitrogen or fixed and prepared as described in the methods below